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BMP signaling orchestrates photoreceptor specification in the zebrafish pineal gland in collaboration with Notch
How is information from multiple signalling cascades integrated in individual cells during development? The group directed by Patrick Blader has shown in the June 1st edition of the journal Development that the regulation of the competence of cells to respond to signalling molecules is itself regulated by the crosstalk between signalling pathways (Quillien et al., 2011).
Structure atomique d’une protéine clé dans la formation des microtubules et essentielle pour l’assemblage du fuseau pendant la division cellulaire.
deux équipes de recherche CNRS/Université Paul Sabatier (L. Mourey, IPBS et A. Merdes, CBD) à Toulouse ont résolu la structure atomique d’une protéine clé dans la formation des microtubules et essentielle pour l’assemblage du fuseau pendant la division cellulaire.
Bienvenue aux nouveaux membres du CBD
BIENVENUE Etudiants en M2R : BONNET Frédéric (eq Pituello) PRINCE Elodie (Eq Bourbon) LY Ismaïla (eq Merdes) Etudiants en Thèses : LAUSSU Julien (eq- Davy) LOURADOUR Isabelle ( Eq Vincent-Crozatier) MILLER Marion ( Eq Haenlin-Waltzer) DELERUE Thomas (Eq Belenguer) Chercheurs recrutés aux Concours CR : BATAILLE Laetitia CR2 INSERM CSS2 ROUSSIGNE Myriam CR1 CNRS (...)
Dual role for Insulin/TOR signaling in the control of hematopoietic progenitor maintenance in Drosophila
Billel Benmimoun, Cédric Polesello, Lucas Waltzer and Marc Haenlin. Development (2012) 139:1713-1717. The interconnected Insulin/IGF signaling (IlS) and Target of Rapamycin (TOR) signaling pathways constitute the main branches of the nutrient-sensing system that couples growth to nutritional conditions in Drosophila. Here, we addressed the influence of these pathways and of diet restriction on the balance between the maintenance of multipotent hematopoietic progenitors and their (...)
NEUROG2 drives cell cycle exit of neuronal precursors by specifically repressing a subset of cyclins acting at the G1 and S phases of the cell cycle
Marine Lacomme, Laurence Liaubet, Fabienne Pituello et Sophie Bel-Vialar Centre de Biologie du Développement, CNRS UMR5547, Toulouse, France 31062 Toulouse, Laboratoire de Génétique Cellulaire, INRA UMR444, Castanet Tolosan France
DSulfatase-1 fine-tunes Hedgehog patterning activity through a novel regulatory feedback loop
Sulfs are secreted sulfatases that catalyse removal of sulfate from Heparan Sulfate Proteoglycans (HSPGs) in the extracellular space. These enzymes are well known to regulate a number of crucial signalling pathways during development. In this study, we report that DSulfatase-1 (DSulf1), the unique Drosophila Sulf protein, is a regulator of Hedgehog (Hh) signalling during wing development. DSulf1 activity is required in both Hh source and Hh receiving cells for proper positioning of Hh (...)
CNRS-INSB: Le code secret de l’œstradiol
Juin 2011
Ceschin D. et al. Methylation specifies distinct estrogen-induced binding site répertoires of CBP to chromatin.
Genes Dev. 2011 Jun 1;25(11):1132-46
Overexpression of EfnB2 in endothelial cells rescue early embryonic lethality
Genetic studies have shown that ephrin-B2 and its cognate EphB4 receptor are necessary for normal embryonic angiogenesis. Moreover, there is overwhelming evidence that ephrin-B2 is involved in tumor vascularization, yet its role in adult angiogenesis has been difficult to track genetically. Here we report the generation of transgenic mice that over-express EfnB2 specifically in endothelial cells (ECs). We show that exogenous expression of EfnB2 under the control of the Tie2 (...)
Stéphanie Bras, Séverine Martin-Lannerée, Vanessa Gobert, Benoît Augé,Osman Breig, Matthieu Sanial, Masamitsu Yamaguchi, Marc Haenlin,Anne Plessis, and Lucas Waltzer (2012). Myeloid leukemia factor is a conserved regulator of RUNX transcription factor activity involved in hematopoiesisPNAS 2012 ; published ahead of print March 12, 2012,
Defining the function of the genes that, like RUNX1, are deregulated in blood cell malignancies represents an important challenge. Myeloid leukemia factors (MLFs) constitute a poorly characterized family of conserved proteins whose founding member, MLF1, has been associated with acute myeloid leukemia in humans Defining the function of the genes that, like RUNX1, are deregulated in blood cell malignancies represents an important challenge. Myeloid leukemia factors (MLFs) constitute a (...)
Partially redundant proneural function reveals the importance of timing during zebrafish olfactory neurogenesis
The expression of transcription factors during developmental processes varies but the importance of the length of time a given transcription factor is expressed during a given developmental process is unclear? This question is addressed in a paper form the Blader group in the latest edition of the journal Development (Madelaine et al., 2011).
Size control of the Drosophila hematopoietic niche by bone morphogenetic protein signaling reveals parallels with mammals
Delphine Pennetier, Justine Oyallon, Ismaël Morin-Poulard, Sébastien Dejean, Alain Vincent, and Michèle Crozatier (2012). PNAS The Drosophila melanogaster larval hematopoietic organ, the lymph gland, is a model to study in vivo the function of the hematopoietic niche. A small cluster of cells in the lymph gland, the Posterior Signaling Centre (PSC), maintains the balance between hematopoietic progenitors (pro-hemocytes) and their differentiation into specialised blood cells (hemocytes). Here, (...)
The RNA-binding protein Xp54nrb isolated from a Ca2+-dependent screen is expressed in neural structures during Xenopus laevis development
ISABELLE NEANT, NINA DEISIG, PIERLUIGI SCERBO, CATHERINE LECLERC and MARC MOREAU. Int. J. Dev. Biol. 55: 923 - 931 (2011) . In amphibian embryos, calcium (Ca2+) signalling is a necessary and sufficient event to induce neural fate. Transient elevations of [Ca2+]i are recorded in neural tissue precursor cells in whole embryos during gastrulation. Using a subtractive cDNA library between control ectoderm (animal caps) and ectoderm induced toward a neural fate by Ca2+ release, we have (...)
Vulgarisation Scientifique au CBD les jeudi 4 et vendredi 5 avril 2013
Deux types de visite ont eu lieu : celle de professeurs du secondaire sur une journée de formation continue intitulée "Profs en labo" proposée par le rectorat ainsi que celle d’élèves de seconde du lycée de Fronton dans le cadre d’un projet bi-disciplinaire Math-Biologie. Ce projet a débuté par une série de travaux pratiques sur la drosophile au lycée alliant génétique et statistiques et s’est finalisé par la visite d’un laboratoire de recherche en Biologie (CBD) et en Mathématiques (IMT) à l’Université (...)
Poste de Maître de conférence affecté au CBD
Profil du poste " Analyse moléculaire du développement animal". Filières de formation concernées : Biologie du Développement : Licence et Master. Biologie Moléculaire : Master. Ojectifs pédagogiques et besoin d’encadrement: Biologie du Développement : du fonctionnel au moléculaire. Le candidat sera amené à participer aux enseignements dépendants du département de Biologie et Géosciences de la nouvelle Faculté des Sciences et de l’Ingénierie (FSI). Le MCF nouvellement recruté enseignera la (...)
The RNA-binding protein ELAVL1/HuR is essential for mouse spermatogenesis, acting both at meiotic and postmeiotic stages
Posttranscriptional mechanisms are crucial to regulate spermatogenesis. Accurate protein synthesis during germ cell development relies on RNA binding proteins that control the storage, stability, and translation of mRNAs in a tightly and temporally regulated manner. Here, we focused on the RNA binding protein Embryonic Lethal Abnormal Vision (ELAV) L1/Human antigen R (HuR) known to be a key regulator of posttranscriptional regulation in somatic cells but the function of which during (...)
RNA binding proteins, RNA granules and gametes: Is unity strength?
Changes in mRNA translation and degradation represents post-transcriptional processes operating during gametogenesis and early embryogenesis to ensure regulated protein synthesis. Numerous mRNA binding proteins (RBPs) have been described in multiple animal models that contribute to the control of mRNA translation and decay during oogenesis and spermatogenesis. An emerging view from studies performed in germ cells and somatic cells is that RBPs associate with their target mRNAs in (...)
Nouvel article
Marc Moreau et Jacques Haiech (Strasbourg) éditeurs d’un numéro spécial consacré au calcium de la revue Biochimie. (voir http://www.sciencedirect.com/scienc...)
The CDC25B phosphatase shortens the G2-phase of neural progenitors and promotes efficient neuron production
Emilie PECO, Timothé ESCUDE, Eric AGIUS, Virginie SABADO, François MEDEVIELLE, Bernard DUCOMMUN, and Fabienne Pituello. Development in press. During embryonic development, changes in cell cycle kinetics have been associated with neurogenesis. This observation suggests that specific cell cycle regulators may be recruited to modify cell cycle dynamics and influence the decision between proliferation and differentiation. In the present study, we investigate the role of core positive cell cycle (...)
Combinatorial coding of Drosophila muscle shape by Collier and Nautilus
by Jonathan Enriquez, Mathilde de Taffin, Michèle Crozatier, Alain Vincent, and Laurence Dubois. Developmental Biology, in press. The genetic and molecular mechanisms that build up muscle diversity are still poorly understood. Muscle diversity is first revealed by the unique patterns of identity transcription factors that accompany muscle progenitor segregation and specification. J. Enriquez et al. have addressed the question of, when and how, a combinatorial code of identity transcription (...)
Post-doctoral position in Drosophila hematopoiesis; deciphering the niche function
A 3 years post-doctoral position is available in Toulouse (France) in the group of Michèle Crozatier and Alain Vincent at “Centre de Biologie du Développement” (http://www-cbd.ups-tlse.fr/spip.php...). One main topic of the group concerns Drosophila hematopoiesis, the hematopoietic niche function and the plasticity of cell fates in the larval hematopoietic organ, the lymph gland. Location : Centre de Biologie du Développement, UMR 5547 (http://www-cbd.ups-tlse.fr/), is a research institute run (...)
jeudi 25 Octobre de 9h à 17h: Congrès "1er Rencontre des Unités de la FRBT" au grand auditorium de l’ UPS
Cette journée sera l’occasion d’une premiere rencontre de tous les intervenants des laboratoires de la Fédéreation de Recherche en Biologie de Toulouse. Cette journée sera l’occasion d’une premiere rencontre de tous les intervenants des laboratoires de la Fédéreation de Recherche en Biologie de Toulouse. Des presentations de chaque laboratoire prévues de 9h a 17 h avec une sessoin poster ou chaque équipe pourra présenter son travail et ses (...)
Tup/Islet1 integrates time and position to specify muscle identity in Drosophila.
Hadi Boukhatmi, Jean Louis Frendo, Jonathan Enriquez, Michèle Crozatier, Laurence Dubois and Alain Vincent (2012) Development 139, 3572-3582. The LIM-homeodomain transcription factor (TF) Tailup/Islet1 (Tup) is a key component of cardiogenesis in Drosophila and vertebrates. In the October issue of Development, http://dev.biologists.org/content/1..., we report another major role for Drosophila Tup/Islet1, in specifying skeletal muscle identity. Interaction between Tinman (Nkx2.5), Tup/Islet1 (...)
Sulfatase 1 promotes the motoneuron to oligodendrocyte fate switch by activating Shh signalling in Olig2 progenitors of the embryonic ventral spinal cord
Yacine Touahri, Nathalie Escalas, Bertrand Benazeraf, Philippe Cochard, Cathy Danesin and Cathy Soula. Journal of Neuroscience (2012) In the developing ventral spinal cord, motor neurons (MNs) and oligodendrocyte precursor cells (OPCs) are sequentially generated from a common pool of neural progenitors included in the so called pMN domain characterized by Olig2 expression. Here, we establish that the secreted Sulfatase 1 (Sulf1) is a major component of the mechanism that causes these (...)
« Le CBD organise ses journées scientifiques les 6 et 7 mai 2013 au Muséum d’Histoire Naturelle de Toulouse. Ces journées permettront aux chercheurs du laboratoire de présenter leurs dernieres avancées scientifiques et de discuter sur les perspectives de leurs travaux».
Présentation des avancées scientifiques effectuées et discussion sur les perspectives
Bibonne A, Néant I, Batut J, Leclerc C, Moreau M, Gilbert T. (2012) ]Three calcium-sensitive genes, fus, brd3 and wdr5, are highly expressed in neural and renal territories during amphibian development. Biochim Biophys Acta.
Numerous Ca2+ signaling events have been associated with early development of vertebrate embryo, from fertilization to organogenesis. In Xenopus laevis, Ca2+ signals are key regulators in the earliest steps of the nervous system development. If neural determination is one of the best-characterized examples of the role of Ca2+ during embryogenesis, increasing literature supports a determining role of organogenesis and differentiation. In blastula the cells of the presumptive ectoderm (animal (...)
Kioske Dimanche 3 Mars
En préambule à la semaine du cerveau qui se déroulera du 11 au 17 Mars, le Kiosque Actus du 3 Mars sera consacré à deux modèles biologiques qui aident à mieux comprendre certains aspects du cerveau : l’abeille pour l’étude de l’odorat et la souris pour l’étude de la mémoire. C’est Dimanche, c’est gratuit.
Ephrin-B1 maintains apical adhesion of neural progenitors (2013) Dina N. Arvanitis, Annie Béhar, Petra Tryoen-Tóth, Jeff O. Bush, Thomas Jungas, Nicolas Vitale and Alice Davy. Development.
Apical neural progenitors are polarized cells whose apical membrane is the site of cell-cell and cell-extracellular matrix adhesion events that are essential to maintain the integrity of the developing neuroepithelium. Apical adhesion is important for several aspects of the nervous system development including morphogenesis and neurogenesis, yet the mechanisms underlying its regulation remain poorly understood. Herein, we show that ephrin-B1, a cell surface protein that engages in cell (...)
Ambre M. Bertholet, Aurélie M.E. Millet,Oriane Guillermin, Marlène Daloyau, Noélie Davezac, Marie-Christine Miquel and Pascale Belenguer (2013). OPA1 loss of function affects in vitro neuronal maturation. BRAIN
Mitochondrial dynamics control the organelle’s morphology, with fusion leading to the formation of elongated tubules and fission leading to isolated puncta, as well as mitochondrial functions. Recent reports have shown that disruptions of mitochondrial dynamics contribute to neurodegenerative diseases. Mutations of the inner membrane GTPase OPA1 are responsible for type 1 dominant optic atrophy (DOA), by mechanisms not fully understood. In murine cortical primary neurons, we show here that (...)
