Dr Henri-Marc Bourbon, Centre de Biologie du Développement, 118 Route de Narbonne, 31062 Toulouse, Phone :(33) 05 61 55 82 88, e-mail : bourbon cict.fr
Transcriptional control of morphogenesis and cell differentiation
In metazoans, cell-specific transcription of most developmental genes is tightly regulated through complex cis-regulatory modules (CRMs), which typically include binding sites for four to eight different regulators. The multiprotein Mediator (MED) complex has the unique ability to interact with multiple sequence-specific transcription factors (TFs) as well as with the RNA polymerase II (PolII) enzyme, and as such is though to act as a malleable scaffold conveying regulatory signals to the basal transcriptional PolII machinery. Though many TFs are known to contact specific MED subunits, the functional consequences of their combinatorial binding on Mediator activity have not been studied in vivo. We are dissecting the underlying molecular mechanisms, using the regulatory CDK8 module as an entry point and Drosophila development as a read out of MED activity.
Gobert, V., Osman, D., Bras, S., Augé, B., Boube, M., Bourbon, H.M., Horn, T., Boutros , M., Haenlin, M. and Waltzer, L. (2010). A genome-wide RNAi screen in Drosophila identifies a differential role of the Mediator CDK8 module subunits in GATA/RUNX-activated transcription. Mol Cell Biol, 30, 2837-48. Kumar, R., Bourbon, H.M. and Massy, B. (2010). Functional conservation of Mei4 for meiotic DNA double-strand break formation from yeasts to mice. Genes and Dev, 24, 1266-80. Makki, R., (...)
Henri-Marc Bourbon, DR CNRS; David Cribbs, PR UPS; Muriel Boube, CR CNRS; Christian Faucher, MCF UPS; Sandra Bernat-Fabre, Research assistant CNRS; Julien Favier, ITAOS, UPS; Aissette Baanannou, PhD student; Clément Immarigeon, PhD student; Luis Humberto Mojica Vazquez, PhD student. Elodie Prince, M2R student